Mechanisms of TRH-induced GH Release (Paradoxical Response) in Human Somatotroph Adenoma Cells

[摘要] References(14)Cited-By(3)The mechanisms of paradoxical TRH response in human somatotroph adenoma cells were investigated using intracellular calcium measurement and static incubation assay. Intracellular calcium measurement revealed that TRH induces a biphasic response: a transient increase followed by a sustained plateau. The transient phase was due to the calcium release from IP3-regulated intracellular calcium store and the subsequent sustained phase was due to the calcium influx through the voltage-gated calcium channels. The signal transduction mechanism of the calcium plateau involved protein kinase C. These calcium responses, especially the second phase, was responsible for the TRH-induced GH release.

[学科分类] 内分泌与代谢学

[关键词] Paradoxical response;TRH;Somatotroph adenoma cell;PKC;GH secretion