The influence of S. frutescens on adrenal cytochrome P450 11B-hydroxylase

[摘要] ENGLISH ABSTRACT:This study:1. describes the preparation of a methanol extract of Sutherlandia frutescens and the HPLCfractionation of the methanol extract.2. investigates the influence of S. frutescens on the binding properties of mitochondrialcytochrome 11 -hydroxylase (CYP11B1) to deoxycorticosterone (DOC) and deoxycortisol,demonstrating that methanol extracts of S. frutescens inhibit the Type I substrate-induceddifference spectra.3. investigates the influence of S. frutescens on the catalytic activity of CYP11B1 expressed inCOS1 cells, demonstrating that the methanol extract of S. frutescens inhibits the conversionof DOC and deoxycortisol.4. describes the sequential extraction of the methanol extract of S. frutescens using organicsolvents and the inhibition of the conversion of DOC by CYP11B1 expressed in COS1 cellsin the presence of these extracts.5. describes the inhibition of the binding of DOC to CYP11B1 in ovine adrenal mitochondria,and the conversion of DOC by CYP11B1 expressed in COS1 cells by these fractions.6. identifies the presence of the flavonoid compounds, orientin vitexin and rutin, in S.frutescens.7. investigates the influence of the flavonoid compounds on the binding of DOC to CYP11B1and on the catalytic activity of DOC by CYP11B1 expressed in COS1 cells.8. identifies the presence of the triterpenoid, sutherlandioside A (SU1), in S. frutescens extractsand investigates its effect on the binding of DOC to CYP11B1.

[发布机构] Stellenbosch University