Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives

[摘要] ENGLISH ABSTRACT:Biotechnological applications are useful for adding value to the local medicinal plantsand may provide an alternative source of pharmacologically-active compounds thusassisting with the conservation of wild populations. Salvia africana-lutea aromatic herbhas long been used in folk medicine by traditional healers in the Western Cape Province(South Africa) for various ailments. As an in vitro conservation strategy, a continuous S.africana-lutea shoot culture was successfully established in solid MS medium containingBA (0.5 mg L-1) and NAA (0.2 mg L-1). The regeneration rate of the S. africana-luteaplants was high which produced approximately 720 plantlets in 20 culture bottles over afour week cycle. The microshoots were rooted in the MS medium without PGRs prior toacclimatisation. A survival rate of 92% was recorded for the greenhouse-acclimatisedshoots.

[发布机构] Stellenbosch University