Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose

[摘要] The study had two main objectives:1) to investigate specific enzyme activity profiles at various developmentalstages and to determine possible implications for sucrose metabolism,2) to incorporate enzyme activity data of different internodes to obtain adetailed model of every stage in the tissue maturation process.The most significant findings of the regulation of sucrose accumulation in thisstudy are centred on three main point controls in sucrose metabolism pathway.Firstly, the maturation of sugarcane internodes coincided with an increase ofSPS in most genotypes, and this underlines the key role of this enzyme insucrose accumulation. Secondly, SuSy activity (cleavage reaction) correlatednegatively with sucrose concentration and hence with tissue maturation process,in most of the varieties. This finding indicates that SuSy could well be implicatedin sucrose metabolism. Thirdly, in vitro PFP activity was found to be negativelycorrelated to sucrose content in sugarcane varieties differing in amount ofsucrose.

[发布机构] Stellenbosch University