Success rates inequine sperm cryopreservation are lower than in other domestic species. Theobjective of this study was to verify the effect of adding 0.1 IU/mL, 1 IU/mLand 10 IU/mL insulin to conventional frozen extender using computerized analysisof sperm motility (CASA), plasma membrane functionality by hypo-osmotic shockand acrossomal membrane integrity through the FITC/PSA assay. No differencewas observed in treatment with 0.1 and 1 IU/mL after thawing in the analysisof motility and kinematic sperm parameters. However, when 10 IU/mL insulin wasused there was a reduction in these parameters.
