The objective ofthis study was to trace the inclusion of bovine meat and bones meal in dietsof laying hens analyzing eggs and theirs fractions (yolk and albumen), by carbonand nitrogen stable isotopes, as well as to evaluate the detectable analyticalminimal index. Two hundred and forty (240) Shaver White laying hens aging 73weeks were distributed in a completely randomized design, with five treatmentsand six replicates. Five increasing levels (0; 1.5; 3.0; 4.5 and 6.0%) of bovinemeat and bones meal in a diet based on corn and soybean meal were evaluated.On the 35th day, 24 eggs per treatment were randomly collected, twelvefor yolk and albumen sampling and another twelve for egg (yolk + albumen) sampling.The isotopic results were analyzed in a multivariate analysis of variance. Throughan error matrix (95% confidence) the ellipses were determined to identify thedifferences between results obtained with supply of experimental diets and controldiet, without 3% bovine meat and bones meal. In egg and yolk, from the isotopespair of the diet with 3% bovine meat and bones meal, there was differentiationin the pair of the control treatment, whereas in the albumen it was possibleto detect the 1.5 bovine meat and bones meal. The stable isotopes techniqueis able to trace the use of bovine meat and bones meal in laying hens feed;the minimal traceable level of inclusion is 1.5% in the albumen and 3.0% inthe egg and yolk.
