The objective of this work was to characterize diploid species, including Lotusjaponicus model species and recombinant inbred lines of the Lotusgenus for resistance to aluminum (Al) toxicity by using acid soil and nutrientsolution. The experiments were conducted in greenhouse, being tested three diploidspecies (L. japonicus MG-20 and GIFU, L. filicaulis and L.burtii), and 180 recombinant inbred lines. In the experiments with diploidspecies, alfalfa was used as a check, and in the experiments with recombinantinbreed lines, GIFU model species was used. In the soil experiments, the morphologicalcharacteristics of the aerial part and the root were evaluated, and in the nutrientsolution experiment, only the root length and growth were evaluated. MG-20 andGIF model species were the most productive in general. The results in nutrientsolution followed the response pattern similar to the one observed in acid soilevaluations. The MG-20 genotype was superior to the others in all Al concentrationstested. Of the 180 recombinant inbred lines tested, 24 were superior and 39inferior to the GIFU species. The great diversity observed in the model speciesand in the recombinant inbred lines can help in future selection of cultivatedgenotypes (tetraploids) inasmuch as they have a group of developed molecularmarkers which can be used at identification of regions responsible for higheror lower tolerance to aluminum toxicity.
