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Effect of oil sources supplementation levels of E vitamin in the diet on the boar semen cryopreservation
[摘要]

The objective ofthis work was to evaluate the effects of addition of oil and of levels of supplementationof E vitamin on the boar sperm cryopreservation. It was used 24 Dalboar 85 boars,at 12 to 18 months, distributed in a complete random design, in a 2 × 3factorial arrangement, with two source of oil (soybean and salmon) and threelevels of E vitamin (150, 300 and 450 mg/kg). Semen was collected weekly (1collection/male), diluted in centrifuge media (1:1) and kept at 25ºC for 2 hours.After centrifugation (800 g, 10 minutes), the pellet was suspended in freezingmedium for the final concentration of 1 × 109 sperm/mL. Thesemen was packaged into 0.5 mL straws and placed inside a test tube (17ºC, 35minutes). After this period, the straws were removed from the tube and keptfor 25 minutes more, submitted to nitrogen vapor for 20 minutes and then dipinto liquid nitrogen. After thawing, the semen of animals fed the ration addedwith soybean oil reduced motility, vigor, percentage of live sperm and HOST.The average motility of the semen after dilution and during cryopreservationprocess was 85 and 84% for the animals fed salmon and soybean oil, respectively.However, after thawing, the motility of the sperms of animals fed ration withsalmon and soybean oil was reduced to 30 and 24%, respectively. Semen of animalstreated with soybean oil presented lower percentage of live sperm and normalmorphology when compared to the ones treated with salmon oil. Supplementationof salmon oil in diet for swines reduces the negative traits observed in thawedsemen of boars.

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[效力级别]  [学科分类] 兽医学
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