The objectivesof this study were to validate a non-extractive RIA for seminal testosteroneand quantify the hormone using a solid-phase commercial kit, and study the correlationbetween testosterone in seminal plasma and sperm characteristics. Parallelismshowed a correlation index r = 0.992 (Y = -5.47 + 1.073X; R2 = 0.985),indicating that the non-extractive method presented is indicated particularlyfor assessment of testosterone when establishing comparisons between samples.Overall mean (±SD) of testosterone level was 0.60±0.65 ng/mL. Correlationwas only found between the seminal concentrations of testosterone and pH ofthe semen.
