An investigation into the stress relieving properties of Sutherlandia frutescens : inhibition of steroidogenic cytochrome P450 enzymes

[摘要] ENGLISH ABSTRACT:This study:I. Investigates the influence of S. frutescens on the binding properties of cytochromeP450-dependent enzymes in ovine adrenocortical mitochondria and microsomes,demonstrating that S. frutescens extracts elicit difference spectra and inhibit theType 1 difference spectra induced by natural steroids.II. Indicates inhibition by S. frutescens extracts of the catalytic activity ofcytochrome P450-dependent-17a-hydroylase and cytochrome P450-dependentsteroid-21-hydroxylase enzymes in ovine adrenocortical microsomes.III. Describes an assay determining the inhibitory effects of S. frutescens, in COS Lcells, on individual cytochrome P450-dependent enzymes - ovine, baboon andhuman cytochrome P450-dependent- L7a-hydroylase, and bovine cytochromeP450-dependent-21-hydroxylase enzymes.IV. Demonstrates that the inhibition of elevated plasma glucocorticoid levels in ratsexposed to chronic immobilization stress could possibly be attributed to theinfluence of hydrophilic and hydrophobic compounds in S. frutescens oncytochromes P450-depndent enzymes.

[发布机构] Stellenbosch University