The objective ofthis trial was to evaluate the chemical composition and fungal microbiota ofdifferent proportions of elephantgrass and mesquite pod silages: 100:0, 67:33,34:66, and 0:100, respectively. A complete randomized design with four replicationsper treatment was used. The material was ensiled in PVC silos and remained sealedduring a 120-days period. After opening the silos, silage samples were collectedfollowed by fungi isolation and identification and determination of the chemicalcomposition of the silages. Addition of mesquite pod to elephantgrass silagedecreased linearly the contents of NDF, ADF, and ash while the opposite wasobserved for DM. A total of 490 colony forming units (CFU) was isolated pergram of material and showed the following distribution: 68, 101, 261, and 58CFU/g of silage, respectively, on 100:0, 67:33, 34:66, and 0:100 treatments.Increasing the proportions of mesquite pod in the silo changed the fungi microbiotaof the silages. For instance, in the treatment containing only mesquite podsilage, a greater diversity of fungi species was observed despite the lowernumber of CFU/g of material. Conversely, the treatment with 66% of mesquitepod showed greater fungi growth and lesser fungi species variety.