Cryopreservation of curimba (Prochilodus lineatus) semen after addition of different diluters, activators and cryoprotectants

[摘要] Semen samples of five specimens of Prochilodus lineatus (Valencienes, 1847) were used to test the toxic and cryoprotectants effects of six solutions based on BTS (Beltsville Thawing Solution®) 4.5%: A - BTS 4.5% + Methanol 10%; B - BTS 4.5% + DMSO 10%; C - BTS 4.5% + KCl 0.072% + Methanol 10%; D - BTS 4.5% + KCl 0.072% + DMSO 10%; E - BTS 4.5% + KI 0.036% + Methanol 10% and F - BTS 4.5% + KI 0.036% + DMSO 10%). The effect of other three activator solutions (Distillated Water, NaHCO3 60 mM and NaHCO3 119 mM) on the rate and duration of sperm motility were also evaluated before and after freezing. No significant differences were observed among the cryoprotectant solutions. Sperm motility rates for P. lineatus were higher for semen activated by NaHCO3 60mM and NaHCO3 119mM, which propitiated the highest sperm motility duration.

[学科分类] 兽医学

[关键词] bicarbonate;curimbatá;DMSO;freezing;methanol;spermatozoids    Introdução A capacidade técnica para preservação de gametas e embriões de peixes e de invertebrados aquáticos tem se expandido rapidamente nos últimos anos;impulsionada primariamente pela indústria aqüícola (Carolsfeld et al