The objective of this research was to evaluate the effect of inclusion (0, 20, 30 and 40%) of whole cottonseed (Gossypium hirsutum) in the diet on chemical composition and fatty acids profile of Santa Inez sheep meat. Twenty four no castrated male sheep were used, (initial 19.0 ± 0.2 kg BW and 4 month old), all kept in confinement regime in individual stalls. The levels of whole cottonseed did not affect chemical composition and the percentages of cholesterol and phospholipids of the lamb meat. However, there was difference among the percentages of the miristic, palmitic and linolenic fatty acids and also to the relationship C18:0 + C18:1 / C16:0. At nutritional point of view, the utilization of whole cottonseed could be recommended, during short periods, up to the level of 40% for finishing animals. In addition, whole cottonseed is a by-product economically viable for presenting low production cost as compared to corn and soybean.
