Design and fabrication of an on-chip PCR device

[摘要] ENGLISH ABSTRACT: An investigation into microfluidic-based PCR (Polymerase Chain Reactiondevices was conducted. A continous-flow serpentine-channel device was fabricated.Channels were etched on a Poly(methyl methacrylate (PMMA substrate using CO2 laser ablation and channels with dimensions 150 x 150µm were obtained. A PMMA lid was bonded via thermal-assisted solvent bondingusing Isopropanol. An in-house external connection schema was fabricated for fluid inlet and outlet ports and pressure driven flow was achieved using a benchtop syringe pump. Notable amplification of a 250bp ( NisinA fragmentwas achieved within 30 minutes, a final concentration of 1.5 µg/L was detected.The relationship between flow rates, temperature zone residency timesand amplification efficiency was examined. This device serves as a proof-ofconcept for two purposes, to show that fabrication of microfluidic devices can be accomplished in low-tech lab spaces and to provide a roadmap towards thedesign of a fully-portable, low cost PCR device.

[发布机构] Stellenbosch University