The optimization of the extraction and purification of horseradish peroxidase from horseradish roots

[摘要] ENGLISH ABSTRACT: This study describes:a) the optimization of the current industrial-scale extraction and purification of Horseradishperoxidase from horseradish at BBI Enzymes, focussing on:a. Raw material quality,b. Extraction,c. Ultra-filtration,d. Salt fractionation,e. Diafiltration,f. Ion Exchange Chromatography,b) developing an new in-process microtitre plate calorimetric assay,c) characterization of main groups of HRP relevant to BBI Enzymes by SDS-PAGE- and HPLCanalysis.

[发布机构] Stellenbosch University