Problem statement: Hypercholesterol and fatty acids in plasma are the main causes forcardiovascular disease. Reduction of risk factors from diet that associated with cardiovascular diseasehas much attention in animals as well as in human. The objective of this study was to investigate theeffects of feeding liquid metabolite combinations produced by five L. plantarum strains on the fattyacids and cholesterol concentration in plasma of postweaning piglets. Approach: A total of 120postweaning piglets aged 26 day olds (Large White x Landrace x Duroc) were randomly assigned intoone of five treatments. (i) basal diet with free antibiotic (-ve control); (ii) basal diet with 0.03% ofchlortetracycline antibiotic (+ve control); (iii) basal diet with 0.3% metabolite of TL1, RG11 and RI11(Com 1); (iv) basal diet with 0.3% metabolite of TL1, RG14 and RS5 (Com 2); (v) basal diet with0.3% metabolite of RG11, RG14 and RI11 (Com 3). The experiment was conducted for 5 weeks. Fattyacids were analysed by Gas Chromatography (GC) and cholesterol was detected using commercialdiagnostic kit. Results: The piglets fed with metabolite combinations were found to reduce plasmacholesterol and Saturated Fatty Acids (SFA) concentrations, particularly in Com 2 group which wassignificantly lower (p<0.05) than the -ve control group. In contrast, the Unsaturated Fatty Acids(USFA) were significantly higher (p<0.05) in Com 2 than -ve control group. The ratio of USFA andSFA was significantly higher (1.14%) in Com 2 as compared to -ve control group. However, there wassignificantly lower (p<0.05) in Com 3 as compared to control groups for the ratio of omega-6 andomega-3 in plasma of piglets. Conclusion: Metabolite combinations produced by L. plantarum strainshave potential effects in influencing the lipid contents and reducing the cholesterol profile of the pig
