EXPLORATION OF MEDICINAL PROPERTIES OF Macrotyloma uniflorum – AN IMPORTANT GRAIN LEGUME CROP PLANT

[摘要] Use of plants in treatment of many diseases has been increased tremendously for enhancement of spectra of plant based natural drugs. So, in this view the present research explore the presence of different phytochemicals, antibacterial, antioxidant and anticancer property of extracts isolated from different parts of Macrotyloma uniflorum. Optimized various biophysical parameters for efficient extraction using Soxhlet and evaluated by antibacterial activity. Extracts extracted with 80% methanol at 75˚C for 12 Soxhlet cycles and at their natural pH shows maximum zone of inhibition with 10.1-28.6 mm diameter. MIC ranges from 1.5-4.5 mg/ml against different bacterial strains. Among the different bacterial strains tested, Gram negative bacteria showed higher susceptibility as compare to Gram positive bacteria. Extracts evaluated for the various important phytochemicals found to contained alkaloids, saponins, tannins, flavonoids, steroids and phenols. The antioxidant activity evaluated by different methods such as DPPH, FRAP, DMPD and hydrogen peroxide radical scavenging, among the different extracts seed coat extract shows best antioxidant activity with 73%, 76%, 74% and 70% at the concentration of 100 mg/ml respectively. Among the anticancer activity checked by SRB Assay against human renal cell adenocarcinoma cancer cell line 786-O, the seed coat extract exhibits highest anticancer potential with 66.2% viability of cells. Overall, the results show that the Macrotyloma uniflorum have various medicinally important properties and it could be not only used as functional food but also the source of new medicines that may be used to treat various disorders caused due to bacterial infections or oxidative stresses.

[学科分类] 生物科学（综合）

[关键词] Antibacterial;Anticancer;Antioxidant;Macrotyloma uniflorum;Phytochemicals;Plant extract.