GENE EXPRESSION PROFILING OF HEPG2 CELLS AFTER TREATMENT WITH BLACK TEA POLYPHENOLS

[摘要] This study aimed to determine the effects of black tea polyphenols on gene expression in hepatocellularcancer cells. The total RNA from HepG2 hepatocellular cancer cells treated with black tea polyphenols was subjected toHuman 14K cDNA microarray analysis. Real-time PCR and Western blot analysis were conducted to verify microarraydata. Black tea polyphenols treatment at the dose of 20 mg/L, 40 mg/L or 80 mg/L for one to three days inhibited thegrowth of HepG2 cells in a dose and time dependent manner. A total of 48 genes showed more than two-fold changeafter black tea polyphenols treatment, including 17 upregulated genes and 31 downregulated genes, and they wereinvolved in the regulation of cell growth, cell cycle, apoptosis, signaling, angiogenesis, tumor invasion and metastasis.Real-time PCR analysis of the selected genes showed that their mRNA expression changes were consistent with themicroarray data. In addition, Western blot analysis of the selected genes showed that their protein expression changeswere consistent with mRNA expression. In conclusion, gene expression profiles provide comprehensive molecularmechanisms by which black tea polyphenols exerts growth inhibition effects on cancer cells. The novel moleculartargets identified in this study may be further exploited as therapeutic strategies for hepatocellular cancer.

[学科分类] 仪器

[关键词] Black tea polyphenols;Microarray;HepG2 cells;Gene expression