In vitro evaluation of cytotoxicity and cellular uptake of alternating copolymers for use as drug delivery vehicles

[摘要] (cont.) It is possible that this aggregation was allowing a portion of the folate ligands to be hidden in the aggregate and unavailable for binding to the FR, the most likely mechanistic cause of the failure of the folate-containing polymers to demonstrate targeting. The versatility of these polymer constructs allows for continued optimization of a targeting delivery system for drugs and imaging agents as lessons discovered from passed studies are incorporated into the design. Initial in vivo biodistribution studies were begun to explore the behavior of these polymers in mouse models of human cancers. The alternating copolymers used in this study do accumulate in tumors in vivo, comparable to the levels of accumulation observed in the literature. It is desirable to minimize the accumulation in other organs, while maximizing the accumulation in the tumor tissue. Therefore, this preliminary study warrants continued investigation of our polymer platform as a delivery vehicle in vitro and in vivo.

[发布机构] Massachusetts Institute of Technology