0064 Deep-brain Imaging Of Lateral Hypothalamic vGAT Neurons During Sleep

[摘要] IntroductionIn-vivo electrophysiology has identified neurons that are selectively active in waking, NREM, or REM sleep (for review see Shiromani and Peever, 2017). However, this method has limitations. New tools are needed to disentangle the brain circuitry regulating complex behaviors such as sleep. We use deep-brain imaging to measure calcium (Ca2+) gradients associated with excitatory signaling (Tian et al., 2009)(Ghosh et al., 2011) in neurons that contain the vesicular GABA transporter (vGAT). The vGAT neurons are a distinct population juxtapositioned with the neurons that contain orexin or MCH in the lateral hypothalamus (Blanco-Centurion et al., 2018), but their activity in sleep in freely-behaving animals is unknown.

[学科分类] 生理学