Dietary therapy restores glutamatergic input to orexin/hypocretin neurons after traumatic brain injury in mice

[摘要] Study ObjectivesIn previous work, dietary branched-chain amino acid (BCAA) supplementation, precursors to de novo glutamate and γ-aminobutyric acid (GABA) synthesis, restored impaired sleep–wake regulation and orexin neuronal activity following traumatic brain injury (TBI) in mice. TBI was speculated to reduce orexin neuronal activity through decreased regional excitatory (glutamate) and/or increased inhibitory (GABA) input. Therefore, we hypothesized that TBI would decrease synaptic glutamate and/or increase synaptic GABA in nerve terminals contacting orexin neurons, and BCAA supplementation would restore TBI-induced changes in synaptic glutamate and/or GABA.

[学科分类] 生理学

[关键词] sleep;wakefulness;branched-chain amino acids;hypocretin;electron microscopy