NANOCOMPOSITE OF Ag0NPs SUPPORTED ON SIO2 MICROSPHERESINDUCES WOUND HEALING IN EXPERIMENTAL SKIN INCISIONS

[摘要] We investigated the influence of coverage the surface of silica microspheres with silvernanoparticles (Ag0NPs) in to skin wound healing. We prepared silica microspheres, eitherpristine or doped with Ag0NPs that were created after the hydrolysis/condensation oftetraethoxysilane, according to Stöber methodology; these substrates were characterizedthrough spectroscopic and microscopic techniques. The wound healing activity was testedand monitored on male Wistar rats (n=15) during 12 days. Finally, a skin biopsy sampleswere analyzed through hematoxylin-eosin and Masson-trichrome stainings or by immunehistochemical localization of fibroblastic and proliferative cells markers. Silicamicrospheres of 300 nm in size, carrying Ag0NPs of about 1.6-3 nm were synthesized andemployed for skin tissue repairing. The animals that received treatment with pristine orAg-doped silica nanosystems showed similar recovery patterns; nevertheless, the animalstreated with Ag doped microspheres exhibited additional features such as an enlargedpresence of hair follicles, insignificant inflammatory infiltrate and an epithelial thicknessof a lesser extent. Similarly, in the same group an important presence of bloodmicrovessels, fibloblast and proliferating cells were observed. These results suggest thatsilica microspheres coated with Ag0NPs induce significantly better skin wound reparationeffects through the modulation of angiogenic, inflammatory, fibrogenic, and proliferativemechanisms.

[学科分类] 生物技术

[关键词] Wound healing;Ag-nanoparticles;SiO2-microspheres;Inflammation;Angiogenesis.