Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom

[摘要] BackgroundLachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV).

[学科分类] 过敏症与临床免疫学

[关键词] Lachesis muta rhombeata;Metalloprotease;Proteases;Snake venom