Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments

[摘要] A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV–visible microspectroscopy in crystallo, solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15–40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O2 in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.

[学科分类] 数学（综合）

[关键词] ULTRALOW-TEMPERATURE X-RAY CRYSTALLOGRAPHY;XANES;HEME PROTEIN;NEUROGLOBIN;OXYGEN BINDING;CRYO-TRAPPING;CRYSTAL MICROSPECTROSCOPY;NEUROPROTECTION;STRUCTURAL BIOLOGY;CO PHOTOLYSIS;SOAK-AND-FREEZE PRESSURIZATION;STRUCTURE DETERMINATION;PROTEIN ST