Characterization of mycorrhizal fungi of the genusTulasnella (Tulasnellaceae, Basidiomycota)in the genus of orchidsBletiafrom Barranca del Cupatitzio Natural Reserve, Mexico

[摘要] The goal of this study was the identification of mycorrhizal fungi associated with three terrestrial orchids of the genusBletiaRuiz & Pav.:B. roezliiRchb. f.,B. purpurataA.Rich., andB. punctataLex., in Barranca del Cupatitzio Natural Reserve - Michoacán, México - . Thirty-nine strains were isolated and morphologically characterized. Nine strains were selected from the molecular analysis.Bletia punctata , an endemic species of Mexico, showed the lowest variability in mycorrhizal fungi. Morphological analysis showed that 39 isolated strains belong to the ‘ Rhizoctonia -like fungal complex’. According with the tree of Euclidian distances generated by the analysis WARD, all isolates were included into four subgroups, all related to the genusTulasnellaJ.Schröt - which belongs to the ‘ Rhizoctonia -like fungal complex’?. Molecular and phylogenetic analysis of the nine selected strains corroborated the results of the morphological study: the sequences obtained were clustered in four subclades related to species ofTulasnella . Our results indicate that a single species ofBletiafrom a single locality can be associated with different species of mycorrhizal fungi, at least during the adult stage and that the combination of morphological and molecular analyses is a good tool to identify orchid mycorrhizal fungi.

[学科分类] 植物学

[关键词] ITS;Rhizoctonia-like fungal complex;terrestrial orchids;Tulasnella;orchid-fungal partnership