Optimisation of high-quality total ribonucleic acid isolation from cartilaginous tissues for real-time polymerase chain reaction analysis

[摘要] ObjectivesStudies which consider the molecular mechanisms of degeneration and regeneration of cartilaginous tissues are seriously hampered by problematic ribonucleic acid (RNA) isolations due to low cell density and the dense, proteoglycan-rich extracellu

[学科分类] 骨科学

[关键词] Ribonucleic acid;Ribonucleic acid isolation;Nucleus pulposus;Annulus fibrosus;Articular cartilage;Meniscus;Real-time polymerase chain reaction