Optimization of Tribenuron-methyl determination by differential pulse polarography using experimental design

[摘要] Differentialpulsepolarography(DPP)wasappliedforthedeterminationoftheherbicideTribenuron-methyl(TBM).Thisisafirststudyforvariousparametersaffectingthereductionpeakcurrentweresimultaneouslyoptimizedusingexperimentaldesignandtheseresultsaredifferentfromotherreports.Theeffectoffactorssuchasvoltagestep,voltagesteptime,pulseamplitude,pulsetime,samplepH,concentrationofthesupportingelectrolyteandthemercurydropsizewereassessedbymeansofa(27-2)fractionalfactorialdesign.Itwasfoundthattheeffectsandinteractionsoffouroutofsevenfactorsweresignificant.Consequently,acentralcompositedesign(CCD)withfourfactors,i.e.,voltagesteptime,pulseamplitude,pulsetimeandsamplepHwasperformedandanoptimumresponsesurfaceequationwasderived.ThestatisticalparametersofthederivedmodelwereR2=0.95andF=20.38.Thelimitofdetection(LOD),limitofquantification(LOQ),linearrangeandrelativestandarddeviation(RSD)were36μgL−1,119μgL−1,0.07–20mgL−1and0.03,respectively.ThepromisingresultsforthedeterminationofTBMinatracelevelshowtheefficiencyoffactorialdesignandresponsesurfacemethodologiesinoptimizingtheparametersofaDPPmethod...

[学科分类] 分析化学