Free fatty acids profile analysis of alcohol extract of Aconitum taipeicum Hand.-Mazz. with gas chromatography-mass spectrometry

[摘要] AconitumtaipeicumHand.-Mazz.,widelyusedintraditionalChinesepharmaceuticals,hasadualeffectonhumanhealth.Itsalcoholextractisclinicallycontroversialpartlybecauseitschemicalcomponentsremaintobeelucidated.Inthispaper,wereportonanelaboratelydevelopedGC-MSmethodforthedeterminationoffreefattyacids(FFAs)inthealcoholextractofAconitumtaipeicumHand.-Mazz.,basedonalcoholextractionandesterificationwithborontrifluoride-methanol.SixtypesoflongchainFFAswereidentifiedintheprofileanalysis,includingtwoessentialfattyacidstothehumanbody.AllthemajorsixFFAsidentifiedinthealcoholextractwerequantifiedwithnonadecanoicacidasaninternalstandard.TheresultsshowedthatthealcoholextractwasabundantinthreetypesofFFAs,withunsaturatedFFAsamountingto67.42%ofthetotalFFAcontent.Linoleicacid(46.24%,13.89±0.36mgg−1)wasthepredominantfattyacid,followedbypalmiticacidandoleicacid.TheseresultsindicatethattheFFAsintheextractofAconitumtaipeicumHand.-Mazz.contributelittletoitsadversehealtheffect...

[学科分类] 分析化学