Pinpointing oxidative modifications in proteins—recent advances in analytical methods
[摘要] Oxidationisoneofthekeydegradationpathwaysinproteinsandisofrelevancetoanalyzeinawidevarietyofresearchdisciplines.Thetypesofdifferentmodificationsoccurringduringoxidationbyreactiveoxygenspecies(ROS)arealmostasmanyasthenumberofavailableanalyticalmethods.Proteinoxidationinbiologicalsampleshastraditionallybeenanalyzedbyvariousproteincarbonylassays,sometimescombinedwithmassspectrometry(MS)foridentificationofthecarbonylatedproteins.MSisnowincreasinglyusedalsoforthedeterminationoftheexactpositionoftheoxidationintheaminoacidsequence,anapproachthatisaidedbyrecentdevelopmentswithinthefieldofproteomics.ThefollowingreviewsummarizestheeffectsofROSonproteins,describesmethodsforlabelingandseparationofoxidizedproteinsincomplexmixtures,andprovidesinsightintovariousMS-basedmethodstolocalizethemodificationswithintheproteinprimarystructure.Pitfallswiththedifferenttechniquesaregiven,aswellasexamplesfromvariousapplicationswithinbiologicalstudies,proteintherapeutics,plantscience,thefoodindustryandindustrialbiotechnology...
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[效力级别] [学科分类] 分析化学
[关键词] [时效性]
