Detection of biomolecules from solutions with high concentration of salts using probe electrospray and nano-electrospray ionization mass spectrometry
[摘要] Probeelectrosprayionization(PESI)isarecentlydevelopedionizationtechniquewhichusesasolidneedleorwireassamplingprobeandESIemitterinsteadofcapillary.PESIisfreefromthecloggingproblemandithashightolerancetosaltsandurea.Wepresenthereinacomparativestudyoftheprobeelectrosprayionization(PESI)andnano-electrosprayionization(nano-ESI)forthemeasurementofbiomoleculesinthesamplesolutionswithhighconcentrationsofsaltsandurea.OurresultsshowthatPESIcouldprovideequivalentionizationperformancewithnano-ESI,andincertaincasesitcouldbesuperiortonano-ESIforthesampleswithhighconcentration(>100mM)ofsaltsandurea.Therefore,PESIcanbeusefulforthedirectanalysisofcell/tissueextractsandproteindigestionwithoutorreducedproceduresinsamplepurification...
[发布日期] [发布机构]
[效力级别] [学科分类] 分析化学
[关键词] [时效性]
