Functional analysis of G-protein coupled receptors using a new fluorescein lactone-based intracellular calcium indicator
[摘要] CalciumfluxassaysarepreferredmethodsindrugdiscoveryforcharacterizingG-proteincoupledreceptors(GPCRs)andscreeningGPCRagonistsandantagonists.AnewfluorescentCa2+indicatorhasbeendevelopedtoprovideimprovedfluorescence-basedassaysfordetectingintracellularCa2+mobilizationofGPCRtargets.Thisfirstfluoresceinlactone-basedCa2+indicatorhas>100timesfluorescenceenhancementuponcalciumbinding.IthasanintracellularCa2+-inducedfluorescencesignalmorethan∼2foldbrighterthanFluo-4AM,and∼4timesbrighterthanFluo-3AM,thetwomostpopularfluorescentCa2+indicators...
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[效力级别] [学科分类] 分析化学
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