Quantification of ceramide levels in mammalian cells by high performance liquid chromatography coupled to tandem mass spectrometry with multiple-reaction-monitoring mode (HPLC-MS/MS-MRM)
[摘要] Ceramidesplayanimportantroleinavarietyofcellularfunctionsincludingcelldifferentiationandapoptosis,responsestoDNAdamageandstress,andtranscriptionalevents.Detectionofceramidesinmammaliancellsisrequiredformanybiologicalstudies.Here,wereportavalidatedmethodusingLC-MS/MS-MRMonanAgilent6410triplequadrupolemassspectrometertosimultaneouslyquantifysixceramidesextractedfrommammaliancelllysatesfollowinga5minHPLCgradient.Thismethoddemonstratedoutstandingsensitivity,accuracy,reproducibility,andspeedofanalysis...
[发布日期] [发布机构]
[效力级别] [学科分类] 分析化学
[关键词] [时效性]
