SERS labels for quantitative assays: application to the quantification of gold nanoparticles uptaken by macrophage cells
[摘要] LabelsbasedonnoblemetalnanoparticlesandsurfaceenhancedRamanscattering(SERS)openednewopportunitiesfortheultrasensitivedetectionofanalytes.Todate,however,SERSlabelsweremostlyusedforqualitativeanalysis,whileleavinglargelyunexploitedtheirpotentialforultrasensitivequantitativeassays.HerewesynthesizedSERSlabelsbasedongoldnanoparticles(AuNPs)obtainedbylaserablationsynthesisinsolutionandwedevelopedageneralmethodforthecorrelationoftheSERSlabelconcentrationwiththeintensityoftheRamansignal.WesuccessfullyusedthismethodforthequantificationofthenumberofAuNPsuptakenbyPMAdifferentiatedU937macrophagecells.OurworkshowsthatquantitativeultrasensitiveassaysbySERSlabelsarepossibleandpointsoutsomeissuesthatmustbeconsideredwhenperformingthistypeofanalysis...
[发布日期] [发布机构]
[效力级别] [学科分类] 分析化学
[关键词] [时效性]
