Acetonitrile shortage: Use of isopropanol as an alternative elution system for ultra/high performance liquid chromatography
[摘要] Acetonitrileisachoiceofsolventforalmostallchromatographicseparations.Inrecentyears,researchersaroundtheglobefacedacetonitrileshortagethataffectedroutineanalyticaloperations.Researcherstriedtocounterthisshortagebyapplyingmanyinnovativesolutions,includingusingultraperformanceliquidchromatography(UPLC)columnsthatareshorterandsmallerindiameterthantraditionalhighperformanceliquidchromatography(HPLC)columns,thussignificantlydecreasingthevolumeofeluentrequired.AlthoughutilizingUPLCinplaceofHPLCcanalleviatethesolventdemandtosomeextent,acetonitrileisgenerallythoughtofasthesolventofchoiceduetoitsversatility.Inthefollowingcommunication,wedescribeanalternativeeluentsystemthatusesisopropanolinplaceofacetonitrileasanorganicmodifierforroutinechromatographicseparations.WereportherethedevelopmentofanisopropanolbasedUPLCprotocolforG5PAMAMdendrimerbasedconjugatesthatwastransferredtosemi-preparativescaletopurifyfunctionalizedformsofthedendrimer(50mg)...
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[效力级别] [学科分类] 分析化学
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