Mass spectrometric identification and characterization of new toremifene metabolites in human urine by liquid chromatography quadrupole/time-of-flight tandem mass spectrometry (LC-QTOFMS)

[摘要] Urine samples after oral administration of toremifene were investigated by liquid chromatography quadrupole time-of-flight tandem mass spectrometry (LC-QTOFMS) with accurate mass measurement. Two new metabolites were detected in positive full scan and targeted MS/MS experiments and their chemical structures were characterized as: 3-methoxy-4,4′-dihydroxy-toremifene and 4-hydroxy-N-oxide-toremifene. As the two metabolites could be detected in all urine samples during one week after intake, they should be potential biomarkers for monitoring the oral administration of toremifene in doping analysis...

[学科分类] 分析化学