Interaction of members of the heat shock protein‐70 family with the macrophage mannose receptor

[摘要] ThemacrophageMRhasbeenthesubjectofinvestigationforover20years,andseveralimportantphysiologicalfunctionshavebeendescribed.However,themolecularmechanismsthatregulateMRsignalingandtraffickingduringtheseprocessesstillremainelusive.ThefocusofthecurrentpaperwastoidentifypotentialcellularMR‐interactingproteins.AninitialscreenofbindingproteinsinMR‐expressingcellswasperformedusingcoimmunoprecipitation,followedbyidentificationofmatchingpeptidesequencesusingproteomicsandMS.Themajorclassofbindingproteinsidentifiedbelongedtotheheatshockfamilyofproteins.ThespecificinteractionoftheMRwithHSP70familymemberswasvalidatedbyWesternblotanalysis,ligandbindingassays,andintracellularcolocalizationusingconfocalmicroscopy.AdditionalstudiesindicatedthatinhibitionoftheHSPBiPbytreatmentofcellswithEGCGreducedBiPinteractionwithandsurfaceexpressionoftheMR.StudiesofpossiblemotifswithinthecytoplasmictailofthereceptorsuggestedthatajuxtamembranedibasicsequencemaycontributetotheinteractionwithBiP.ThesefindingssuggestthatthemolecularassociationoftheMRwithHSP70familymembersviathereceptorcytoplasmictailmaycontributetoMRtraffickinginmacrophages...

[学科分类] 生理学

[关键词] receptor;binding proteins;cytoplasmic tail