Senescent profile of angiogenic T cells from systemic lupus erythematosus patients

[摘要] Thechronicinflammatoryenvironmentassociatedwithsystemiclupuserythematosuscanleadtoanacceleratedimmunosenescenceresponsiblefortheendothelialdamageandincreasedcardiovascularriskobservedinthesepatients.Thepresentstudyanalyzedtwopopulationswithoppositeeffectsonvascularendothelium,angiogenicTcellsandthesenescentCD4+CD28nullsubset,in84systemiclupuserythematosuspatientsand46healthycontrols.Also,48rheumatoidarthritispatientsand72individualswithtraditionalcardiovascularriskfactorsparticipatedasdiseasecontrols.PhenotypiccharacterizationofCD28+andCD28nullcellswasperformedbyanalyzingmarkersofsenescence(CCR7,CD27,CD57)andcytotoxicity(CD56,perforin,granzymeB,IFN‐γ).IL‐1β,IL‐6,IL‐8,IL‐10,IL‐12,IL‐17A,IFN‐α,IFN‐γ,TNF‐α,Blymphocytestimulator,andGM‐CSFserumlevelswereanalyzedinsystemiclupuserythematosuspatientsandhealthycontrols.CD4+CD28nullcellswerenotablyincreasedinthesystemiclupuserythematosuspatientsanddiseasecontrolscomparedwithhealthycontrols.Incontrast,angiogenicTcellswereonlyreducedinthediseasecontrols(thosewithrheumatoidarthritisortraditionalcardiovascularriskfactors).Nevertheless,ananomalouspresenceofCD28null‐angiogenicTcells,withcytotoxicandsenescentcharacteristics,wasnotedinsystemiclupuserythematosuspatientsinassociationwithanti‐dsDNAtiter,anti‐SSA/RoantibodiesandcirculatingTNF‐α,IL‐8,IFN‐α,andBlymphocytestimulatoramounts.Thissubsetwasalsodetectedinthosewithtraditionalcardiovascularriskfactorsbutnotintherheumatoidarthritispatients.Incontrast,CD28+‐angiogenicTcellswerereducedinthesystemiclupuserythematosuspatientswithcardiovasculardisorders.Inconclusion,CD28expressionmustbeusedtoredefinetheangiogenicTcellpopulation,becauseinpathologicconditions,asenescentCD28null‐angiogenicTcellsubsetwithinflammatory,ratherthanprotective,effectscouldbepresent...

[学科分类] 生理学

[关键词] Tang cells;CD4+CD28null;cardiovascular disease;BLyS;IFN‐α