ЛОКАЛЬНОЕ СУБРЕТИНАЛЬНОЕ ВВЕДЕНИЕ КСЕНОГЕННЫХ СТВОЛОВЫХ КЛЕТОК, МЕЧЕННЫХ МАГНИТНЫМИ ЧАСТИЦАМИ, В ЭКСПЕРИМЕНТЕ

[摘要] Purpose. To develop a technique for local subretinal injection of xenogeneic stem cells labeled with magnetic particles and to prove experimentally its effectiveness.Material and methods. We used a line of stem cells HEK-293 GFP, labeled with magnetic particles. The study was made in 84 eyes of 42 chinchilla rabbits aged 6 months, the weight was from 2.5 to 3.5kg. All right eyes were experimental (42 eyes) and all left eyes (42 eyes) were in the control group. In the experimental group we used original complex of polymer elastic magnetic implant (PEMI) with a laser probe and fixed it to the sclera, then we made a median vitrectomy and injected HEK-293 GFP under the retina using a specially designed dispenser. In the control group PEMI was not fixed. We examined animals using biomicroscopy, ophthalmoscopy, ultrasound scanning, optical coherence tomography (OCT), computer tomography (CT), morphological study (cryo-histological sections) 1, 3, 5, 7, 14 days and 1 month after surgery.Results. According to the results of biomicroscopy in observation periods up to 3 days the vascular injection was visualized in the area operation. According to the results of ophthalmoscopy and ultrasound scanning during the first day the local retinal detachment was visualized in the area of local injection of the stem cells, which was not noted in terms of further observations. CT helped us to confirm the localization of PEMI fixation. The morphological study results showed that cells were located in the subretinal space up to 14 days in the experimental group, and only up 3 days in the control group.Conclusion. The developed surgical technique enables to control the injection of cells into the subretinal space, reduces the risk of tissue damage and exit cells in the vitreous space. The suggested method allows to fix the cellular material in the local place of the injection and enables to predict cells` movement. .

[学科分类] 眼科学

[关键词] stem cells;magnetic particles;subretinal injection;стволовые клетки;магнитные частицы;субретинальное введение