Three-Dimensional Structure of Arabidopsis thaliana Lipase Predicted by Homology Modeling Method

[摘要] Triacylglycerol lipases have been thoroughly characterized in mammals and microorganisms. By contrast, very little is known about plant lipases. In this investigation, a homology model of Arabidopsis thaliana lipase (NP_179126) was constructed using a human gastric lipase (PDB ID: 1HLG), as a template for model building. This model was then assessed for stereochemical quality and side chain environment. Natural substrates: tributyrin, trioctanoin and triolen were docked into the model to investigate ligand-substrate interaction.

[学科分类] 生物技术

[关键词] comparative homology modeling;protein modeling;bioinformatics;lipase enzymes