Motifs, binding, and expression : computational studies of transcriptional regulation

[摘要] (cont.) We next present a biophysically motivated framework for modeling protein-DNA interactions and show how it leads to very natural algorithms for analyzing the binding specificity of an immunoprecipitated protein, and jointly analyzing protein localization data for multiple regulators or multiple conditions. Finally, we present an analysis of transcriptional coregulator binding in a variety of mouse tissues and a method for predicting which proteins form complexes with the coregulator based purely on the sequence of the regions it binds. We detail a simple but powerful model relating regulator binding to gene expression, and show how the position of regulatory regions is of crucial importance for predicting the expression level of nearby genes.

[发布机构] Massachusetts Institute of Technology