Molecular Characterization of the Gene Encoding for the Salivaricin B Activity and its Flanking Sequences

[摘要] Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated. Since they are associated with foods of plant and animal origins, the shared interest is that they can be utilized as a vector to deliver the active constituents at the target in the gastrointestinal tract after digestion and be used in therapy. In this respect, the molecular pattern of the expression and translocation of salivaricin B (SalB), a bacteriocin, from Lactobacillus salivarius M7 was studied at the molecular level. The gene encoding SalB and the flanking sequences were obtained and sequenced. The gene encoding SalB comprised an open reading frame (ORF) of 171 bp having a 57 bp long leader sequence and a 114 bp long structural part. Ribosomal binding site (GAGG, RBS) is located at a cannonical distance of 8 bp upstream from the start site.

[学科分类] 生物科学（综合）

[关键词] Gene;Gene structure;Salivaricin B;PCR;Inverse PCR;Degenerate primers;Lactobacillus salivarius M7.