Immunoassay sensitivity and kinetic enhancement in cell culture media using electrokinetic preconcentration

[摘要] (cont.) Our immunoassay also benefits from sub microliter required sample volume and may be easily integrated directly to microfluidic cell cultures. Furthermore, we fabricated micro electrodes integrated on chip to the electrokinetic preconcentrator to directly measure the electric field which accompanies nonlinear electrokinetic flow. The time response electrical profiles of electrokinetic preconcentration was obtained and correlated to the protein concentration profile to study electrokinetic behavior. Our efforts yielded a method to directly visualize the electric field distribution in nonlinear electrokinetic flow and a direct relationship between the depletion effects of electrokinetic behavior and the electric field profile.

[发布机构] Massachusetts Institute of Technology