Strawberry Extract’s Effects onEnterococcus faecalisandPorphyromonas gingivalis Biofilms in vitro

[摘要] Background :Enterococcus faecalis( E. faecalis ) andPorphyromonas gingivalis( P. gingivalis ) are oral bacteria related to root canal infection and periodontal disease pathogenesis. Strawberries ( Fragaria x ananassa ) fruit are rich in vitamins and minerals, have antibacterial and antioxidant effects.Objective : This study investigated the inhibition effect of strawberry extract on monospecies and multispeciesE. faecalisandP. gingivalisbacteria grown as biofilmsin vitro. Methods:This study usedE. faecalisATCC 29212and P. gingivalisATCC 33277. It analyzed the effect of strawberry extract on bacteria biofilm formation using a biofilm assay on microplate wells. Five concentrations of strawberry extracts were used (100%, 50%, 25%, 12.5%, and 6.25%), and the inhibition effect was observed after a 1h, 3h, 6h, and 24h incubation period. Biofilms without the strawberry extract were used as the negative controls, and crystal violet and safranin (0.5% w / v ) were used to count the biofilm mass. The biofilms grown on microplates were counted using an ELISA reader at 450 nm after 200 mL of 90% ethanol was added to attract the absorbed stain. The strawberry extract inhibition effectiveness on the biofilm formation of each bacterium tested was analyzed using one-way Anova, where p<0.05 was defined as a significant difference.Result : The strawberry extract inhibited the tested monospecies and multispecies bacteria biofilm formation. The optimal strawberry extract concentration for the inhibition of either monospecies biofilms was 100%. However, the optimal incubation time for the strawberry extract to inhibit the multispecies biofilm formation was 24h, which was the study’s biofilm maturity phase.Conclusions:The 100% strawberry extract concentration inhibited the formation of both the monospecies and multispeciesE. faecalisandP. gingivalisbiofilms. Future studies are needed to evaluate the potential of strawberry extract as an alternative dental therapy.

[学科分类] 口腔科学