Characterization of adipose-derived stem cells freshly isolated from liposuction aspirates performed with Prolipostem®

[摘要] Lipofilling is a cosmetic surgical procedure that consists in the withdrawal of a small quantity of fat tissue from a suitable anatomic area and the reimplantation of this tissue in another corporeal district in the same individual, so as to obtain a filling effect. Recently, adipose-derived stem cells (ADSCs) have been isolated in the aspirates. These are pluripotent mesenchymal stem cells that are able to differentiate in mature adipose cells or other adult mesenchymal cells after paracrine or autocrine hormonal stimulations, thus favoring a longer survival of the implanted tissues. In this article, we have defined a new method for liposuction (Prolipostem®), where the ADSCs are recovered and mixed with the suctioned adipose tissue derived from the abdominal fat, before the reimplantation. We have demonstrated by immunocytochemistry the presence of ADSCs in the adipose tissue taken with Prolipostem® from the abdominal fat and the ability of these ADSCs to differentiate in mature adipose cells in vitro. The possibility to enrich the tissue to be implanted with ADSCs would assure a longer survival of the cells implanted and a regeneration of the host tissue thanks to growth and angiogenic stimuli induced by the ADSCs.

[学科分类] 皮肤病学

[关键词] Adipose-derived stem cells (ADSCs);Diaminobenzidine (DAB);Dulbecco’s modified Eagle’s medium (DMEM);Fetal bovine serum (FBS);Phosphate-buffered saline (PBS)