Expression of CAF-Related Proteins Is Associated with Histologic Grade of Breast Phyllodes Tumor

[摘要] Purpose. The purpose of this study was to investigate the expression of cancer-associated fibroblast- (CAF-) related proteins and the implications in breast phyllodes tumor (PT). Methods. Tissue microarrays of 194 PT cases (151 benign PT, 27 borderline PT, and 16 malignant PT) were constructed. We performed immunohistochemical staining for CAF-related proteins (podoplanin, prolyl 4-hydroxylase, FAP?, S100A4, PDGFR ?/?, and NG2) and analyzed the results according to clinicopathologic parameters. Results. Expression of PDGFR? and PDGFR? in the stromal component increased with increasing histologic grade of PT (p=0.003 and p=0.034, resp.). Among clinicopathologic parameters, only expression of FAP? in stroma was associated with distant metastasis (p=0.002). In univariate analysis, stromal expression of PDGFR? was associated with shorter overall survival (p=0.002). In Cox multivariate analysis, stromal overgrowth and PDGFR? stromal positivity were associated with shorter overall survival (p=0.006 and p=0.050, resp.). Furthermore, expression of PDGFR? in stroma was associated with shorter overall survival in patients with malignant PT (p=0.041). Conclusion. Stromal expression of PDGFR? and PDGFR? increased with increasing histologic grade of PT. In addition, PDGFR stromal positivity was associated with shorter overall survival. These results suggest that CAFs are associated with breast PT progression.

[学科分类] 生理学与病理学