Exploring the biological functions of AlkB proteins and how they relate to AAG
[摘要] (cont.) Here we have shown in a mouse model that Abh2 and Abh3 overlap with Aag in protecting mice from sensitivity in response to chemically induced chronic inflammation, in which etheno base lesions are readily generated. In addition, we also employed a biochemical approach using a comprehensive library of lesion-containing DNA oligonucleotides to study the redundancy in repair activity between AAG and AlkB. In doing so, we have found new substrates for AAG and in particular, 1-methylguanine, is a new substrate shared between AAG and AlkB. Thus, although these two proteins employ different mechanisms for repair, our studies established further evidence of the interplay between these proteins and the different repair pathways they represent, underscoring the importance of alkylation damage repair for proper cell homeostasis.
[发布日期] [发布机构] Massachusetts Institute of Technology
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