Optimization of Process Parameters for ?-Polylysine Production by Response Surface Methods

[摘要] ?-Polylysine (?-PL) is a highly safe natural food preservative with a broad antimicrobial spectrum, excellent corrosion resistances, and great commercial potentials. In the present work, we evaluated the ?-PL adsorption performances of HZB-3B and D155 resins and optimized the adsorption and desorption conditions by single-factor test, response surface method, and orthogonal design. The complexes of resin and ?-PL were characterized by SEM and FITR. The results indicated that D155 resin had the best ?-PL adsorption performance and was selected for the separation and purification of ?-PL. The conditions for the static adsorption of ?-PL on D155 resin were optimized as follows: ?-PL solution 40?g/L, pH 8.5, resins 15?g/L, and absorption time 14?h. The adsorption efficiency of ?-PL under the optimal conditions was 96.84%. The ?-PL adsorbed on the D155 resin was easily desorbed with 0.4?mol/L HCl at 30°C in 10?h. The highest desorption efficiency was 97.57% and the overall recovery of ?-PL was 94.49% under the optimal conditions. The excellent ?-PL adsorption and desorption properties of D155 resin including high selectivity and adsorption capacity, easy desorption, and high stability make it a good candidate for the isolation of ?-PL from fermentation broths.

[学科分类] 材料工程