Aminoglycoside susceptibility of Pasteurella multocidaisolates from bovine respiratory infections in China and mutations in ribosomal protein S5associated with high-level induced spectinomycin resistance

[摘要] Twenty-three isolates of Pasteurella multocida were tested forsusceptibility to six aminoglycoside agents and screened by polymerase chain reaction forthe presence of aminoglycoside resistance genes. In addition, mutations in theresistance-determining region of strains showing a high level of induced resistance tospectinomycin strains were examined. Susceptibility testing showed that all of theisolates were resistant to at least two types of aminoglycosides, and that the mosteffective antimicrobial was spectinomycin. The resistance genes aphA1,strB and aacA4 were present in all 23 isolates. In thethree induced spectinomycin-resistant strains, a 9-bp deletion in rpsEthat encodes ribosomal protein S5 was detected.

[学科分类] 兽医学

[关键词] aminoglycoside resistance;mutations;Pasteurella multocida