Defect of rabies virus phosphoprotein in its interferon-antagonist activitynegatively affects viral replication in muscle cells

[摘要] Attenuated derivative rabies virus Ni-CE replicates in muscle cells less efficiently thandoes the parental pathogenic strain Nishigahara. To examine the mechanism underlying theless efficient replication of Ni-CE, we compared the activities of Ni-CE and Nishigaharaphosphoproteins, viral interferon (IFN) antagonists, to suppress IFN-β promoter activityin muscle cells and we demonstrated a defect of Ni-CE phosphoprotein in this ability.Treatment with an IFN-β-neutralizing antibody improved the replication efficiency of Ni-CEin muscle cells, indicating that produced IFN inhibits Ni-CE replication. The resultsindicate the importance of IFN antagonism of rabies virus phosphoprotein for viralreplication in muscle cells.

[学科分类] 兽医学

[关键词] interferon antagonist;muscle cells;phosphoprotein;rabies virus