Validation of an HPLC assay for determination of Telazol in pregnant pigs:application to placental transfer study

[摘要] Anesthetics utilized for the immobilization of pregnant mammals are prone to crossing theplacental barrier and cause adverse effects to the fetuses. In this study, we develop afacile method employing high performance liquid chromatography (HPLC) for the study ofTelazol crossing the placental barrier of pregnant pigs. The method mainly relies on theefficient extraction strategy that includes the mobile phase composed of 10 mM ammoniumacetate aqueous solution-acetonitrile (14, v/v). When the injected dose of Telazol is 10mg/kg (5 mg/kg of each constituent drug, zolazepem and tiletamine), zolazepam can crossthe placental barrier as it is detected in both uterus and umbilical cord withapproximately the same content. Conversely, tiletamine is detected in neither uterus norumbilical cord, indicating the absence of placental transfer of tiletamine. The differentabsorption rates of the two dosage-equal compounds by pigs are found to be the main causeof their different abilities to cross the placental barrier.

[学科分类] 兽医学

[关键词] HPLC;placental barrier;pregnant pigs;tiletamine;zolazepam